Difference between revisions of "Part:BBa K2610002"
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<p>Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. <br> iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) </p> <br> | <p>Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. <br> iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) </p> <br> | ||
| − | [[ | + | [[File:T--Leiden--heatmap.png|Figure 1 caption]] |
<br><img src="https://static.igem.org/mediawiki/2018/9/91/T--Leiden--heatmap.png" max-width="30%"> | <br><img src="https://static.igem.org/mediawiki/2018/9/91/T--Leiden--heatmap.png" max-width="30%"> | ||
<b>Figure 1.</b> Heatmap with stress-activated promoters | <b>Figure 1.</b> Heatmap with stress-activated promoters | ||
Revision as of 12:06, 9 October 2018
pIbpA
Usage and Biology
Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli.
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1)
<img src="
" max-width="30%">
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]