Difference between revisions of "Part:BBa K2886002"
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VEGF-scFv which stands for single-chain antibody fragment of vascular endothelial growth factor is a protein domain that can recognize VEGF.
 
VEGF-scFv which stands for single-chain antibody fragment of vascular endothelial growth factor is a protein domain that can recognize VEGF.
  
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VEGF-scFv was firstly introduced to iGEM in the year   . And this year, we make new improvement to the VEGF-scFv. In our STEP system, to improve the affinity of the VEGF-scFv to VEGF, we used  Rosetta to help design our protein domain. However, we found that the initial version of VEGF-scFv in parts has no accepted structure in PDD (Protein Data Base) of NCBI. So, to improve the reliability of our results, we introduced a kind of VEGF-scFv that has accepted structure in PDD to iGEM, making it more accessible to iGEMers.
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VEGF-scFv was firstly introduced to iGEM by iGEM15_NCTU-Formosa in the year 2015 (BBa_K1694003) . And this year, we make new improvement to the VEGF-scFv. In our STEP system, to improve the affinity of the VEGF-scFv to VEGF, we used  Rosetta to help design our protein domain. However, we found that the initial version of VEGF-scFv in parts has no accepted structure in PDD (Protein Data Base) of NCBI. So, to improve the reliability of our results, we introduced a kind of VEGF-scFv that has accepted structure in PDD to iGEM, making it more accessible to iGEMers.
  
 
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<!-- Add more about the biology of this part here

Revision as of 11:01, 9 October 2018


VEGF-scFv that can recognize the VEGF

VEGF-scFv which stands for single-chain antibody fragment of vascular endothelial growth factor is a protein domain that can recognize VEGF.

VEGF-scFv was firstly introduced to iGEM by iGEM15_NCTU-Formosa in the year 2015 (BBa_K1694003) . And this year, we make new improvement to the VEGF-scFv. In our STEP system, to improve the affinity of the VEGF-scFv to VEGF, we used Rosetta to help design our protein domain. However, we found that the initial version of VEGF-scFv in parts has no accepted structure in PDD (Protein Data Base) of NCBI. So, to improve the reliability of our results, we introduced a kind of VEGF-scFv that has accepted structure in PDD to iGEM, making it more accessible to iGEMers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]