Difference between revisions of "Part:BBa K2886002"

Revision as of 10:55, 9 October 2018

VEGF-scFv that can recognize the VEGF

VEGF-scFv which stands for single-chain antibody fragment of vascular endothelial growth factor is a protein domain that can recognize VEGF.

VEGF-scFv was firstly introduced to iGEM in the year . And this year, we make new improvement to the VEGF-scFv. In our STEP system, to improve the affinity of the VEGF-scFv to VEGF, we used Rosetta to help design our protein domain. However, we found that the initial version of VEGF-scFv in parts has no accepted structure in PDD (Protein Data Base) of NCBI. So, to improve the reliability of our results, we introduced a kind of VEGF-scFv that has accepted structure in PDD to iGEM, making it more accessible to iGEMers.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 524
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 VEGF-scFv which stands for single-chain antibody fragment of vascular endothelial growth factor is a protein domain that can recognize VEGF.
+VEGF-scFv was firstly introduced to iGEM in the year    . And this year, we make new improvement to the VEGF-scFv. In our STEP system, to improve the affinity of the VEGF-scFv to VEGF, we used  Rosetta to help design our protein domain. However, we found that the initial version of VEGF-scFv in parts has no accepted structure in PDD (Protein Data Base) of NCBI. So, to improve the reliability of our results, we introduced a kind of VEGF-scFv that has accepted structure in PDD to iGEM, making it more accessible to iGEMers.
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