Difference between revisions of "Part:BBa K2610002"

 
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===Usage and Biology===
 
===Usage and Biology===
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iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1)
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<img src="https://static.igem.org/mediawiki/2018/9/91/T--Leiden--heatmap.png" max-width="30%">
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Figure 1. Heatmap with stress-activated promoters
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As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.
  
 
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Revision as of 10:29, 9 October 2018


pIbpA

Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]