Difference between revisions of "Part:BBa J45003:Design"
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− | == | + | ==Design Consideration== |
− | + | *Attempted but failed to construct by PCR | |
− | + | ====Forward primer==== | |
+ | <code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G'''at gga ggt aat gcg agt tct tc''' -3'</code> | ||
− | == | + | ====Reverse primer==== |
− | + | <code>5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA'''a ccg gtt cta acg agc gaa ag''' -3'</code> | |
==Source== | ==Source== | ||
− | + | ''JMT'' from ''Arabidopsis thaliana'' ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=13676828 GenBank AY008434]) | |
− | + | ||
==References== | ==References== | ||
− | Seo | + | <biblio> |
+ | #Seo-PNAS-2001 pmid=11287667 | ||
+ | </biblio> | ||
+ | |||
+ | [http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry] |
Latest revision as of 17:35, 10 March 2008
Design Consideration
- Attempted but failed to construct by PCR
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA Gat gga ggt aat gcg agt tct tc -3'
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAa ccg gtt cta acg agc gaa ag -3'
Source
JMT from Arabidopsis thaliana ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=13676828 GenBank AY008434])
References
<biblio>
- Seo-PNAS-2001 pmid=11287667
</biblio>
[http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]