Difference between revisions of "Part:BBa K2804006:Design"
 
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===Design Notes===
 
===Design Notes===
 
The six-histidine tail was added C-terminally as a consideration for future Atomic Force Microscopy real-time analysis of the interaction between the CBD cipA and bacterial cellulose.
 
The six-histidine tail was added C-terminally as a consideration for future Atomic Force Microscopy real-time analysis of the interaction between the CBD cipA and bacterial cellulose.
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It was necessary to run a PCR for adding the basepairs 5'-ccg-3'and 5'-aaaac-3 to the N-terminus and C-terminus of the gBlock and then allow the enzymatic cleavage by EcoRI and PstI
 
 
The sequence was optimized for E. coli.
 
The sequence was optimized for E. coli.
  

Latest revision as of 06:33, 9 October 2018


Superfolder GFP with six-histidine tail


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The six-histidine tail was added C-terminally as a consideration for future Atomic Force Microscopy real-time analysis of the interaction between the CBD cipA and bacterial cellulose. The sequence was optimized for E. coli.


Source

Synthesized from IDT.

References