Difference between revisions of "Part:BBa K2804006:Design"
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===Design Notes=== | ===Design Notes=== | ||
The six-histidine tail was added C-terminally as a consideration for future Atomic Force Microscopy real-time analysis of the interaction between the CBD cipA and bacterial cellulose. | The six-histidine tail was added C-terminally as a consideration for future Atomic Force Microscopy real-time analysis of the interaction between the CBD cipA and bacterial cellulose. | ||
| + | It was necessary to run a PCR for adding the basepairs 5'-ccg-3'and 5'-aaaac-3 to the N-terminus and C-terminus of the gBlock and then allow the enzymatic cleavage by EcoRI and PstI | ||
The sequence was optimized for E. coli. | The sequence was optimized for E. coli. | ||
Revision as of 06:33, 9 October 2018
Superfolder GFP with six-histidine tail
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The six-histidine tail was added C-terminally as a consideration for future Atomic Force Microscopy real-time analysis of the interaction between the CBD cipA and bacterial cellulose. It was necessary to run a PCR for adding the basepairs 5'-ccg-3'and 5'-aaaac-3 to the N-terminus and C-terminus of the gBlock and then allow the enzymatic cleavage by EcoRI and PstI The sequence was optimized for E. coli.
Source
Synthesized from IDT.