Difference between revisions of "Part:BBa K2804002:Design"
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===Design Notes===
 
===Design Notes===
−
The mature active form of BMP2 (114 amino acids) was considered for the design.
+
The mature active form of BMP2 (114 amino acids) was considered.
 +
It was necessary to run a PCR for adding the basepairs 5'-ccg-3'and 5'-aaaac-3 to the N-terminus and C-terminus of the gBlock and then allow the enzymatic cleavage by EcoRI and PstI.
 
The sequence was condon optimized for E. coli
 
The sequence was condon optimized for E. coli
  

Revision as of 06:33, 9 October 2018


Bone morphogenetic protein 2 (BMP2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mature active form of BMP2 (114 amino acids) was considered. It was necessary to run a PCR for adding the basepairs 5'-ccg-3'and 5'-aaaac-3 to the N-terminus and C-terminus of the gBlock and then allow the enzymatic cleavage by EcoRI and PstI. The sequence was condon optimized for E. coli

Source

Synthetized from IDT.

References

McCarthy, B., Yuan, Y., & Koria, P. (2016). Elastin-like-polypeptide based fusion proteins for osteogenic factor delivery in bone healing. Biotechnology Progress, 32(4):1029-37.