Difference between revisions of "Part:BBa K2889003:Experience"

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===Applications of BBa_K2889003===
 
===Applications of BBa_K2889003===
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https://static.igem.org/mediawiki/parts/7/72/Electrophoresis_of_miR-21-sponge-4s_PCR_product.jpg
 
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First we amplified miR-21-sponge-4s using primers. After that, we purified the PCR products by PCR Purification Kit and digested them with restriction enzymes EcoR I and Pst I
 
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https://static.igem.org/mediawiki/parts/5/5b/Digested_the_PSB1C3_vectors_with_EcoRI_and_PstI.jpeg
 
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We digested the PSB1C3 vectors with restriction enzymes EcoRI and PstI
 
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https://static.igem.org/mediawiki/parts/1/1d/MiR-21-sponge-4s_sequencing_map.jpeg
 
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We selected the positive clones by PCR and sequencing
 
  
 
===User Reviews===
 
===User Reviews===

Revision as of 04:47, 9 October 2018


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Please enter how you used this part and how it worked out.

Applications of BBa_K2889003

User Reviews

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