Difference between revisions of "Part:BBa J45300"
 
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<partinfo>BBa_J45300 short</partinfo>
 
<partinfo>BBa_J45300 short</partinfo>
  
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This part is used to generate salicylic acid.  Consists of promoter, RBS, pchBA, terminator.  This part is almost identical to <partinfo>J45320</partinfo>, except that it uses the B0030 RBS instead of B0032 (which is in <partinfo>J45320</partinfo>).  [[Image:J45300.jpg]]
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The salicylic acid generator (<partinfo>BBa_J45300</partinfo>) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate.  The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (<partinfo>BBa_R0011</partinfo>) and an odor precursor enzyme generator (<partinfo>BBa_J45319</partinfo>).  Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.
  
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Salicylic Acid Generating Device (SAGD):
 
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*converts chorismate into salicylic acid
 
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*production of salicylic acid was verified by controlled tests: E. coli with WGD but no SAGD did not produce methyl salicylate (that is, they did not smell minty), while E. coli containing both the SAGD and WGD did produce methyl salicylate autonomously.
 
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_J45300</partinfo> is almost identical to <partinfo>BBa_J45320</partinfo> except that it uses a different ribosome binding site (<partinfo>BBa_B0030</partinfo> instead of <partinfo>BBa_B0032</partinfo>).
  
 
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Latest revision as of 20:54, 9 March 2008

Salicylic acid generator

The salicylic acid generator (BBa_J45300) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0011) and an odor precursor enzyme generator (BBa_J45319). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.

Usage and Biology

BBa_J45300 is almost identical to BBa_J45320 except that it uses a different ribosome binding site (BBa_B0030 instead of BBa_B0032).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 391
    Illegal NgoMIV site found at 795
    Illegal NgoMIV site found at 920
    Illegal NgoMIV site found at 931
    Illegal NgoMIV site found at 1212
    Illegal NgoMIV site found at 1680
  • 1000
    COMPATIBLE WITH RFC[1000]