Difference between revisions of "Part:BBa J45700"

Latest revision as of 23:47, 8 March 2008

Wintergreen odor biosynthetic system

We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor.

Usage and Biology

To construct a complete wintergreen odor biosynthetic system, we needed to design an additional biosynthetic device for wintergreen odor precursor production. We designed a biosynthetic device for production of salicylate from the cellular metabolite chorismate (BBa_J45320) based on the pchBA genes from Pseudomonas aeruginosa. The enzyme PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Serino et al. previously demonstrated salicylate formation in E. coli using the pchBA genes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 744
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1689
Illegal NgoMIV site found at 2093
Illegal NgoMIV site found at 2218
Illegal NgoMIV site found at 2229
Illegal NgoMIV site found at 2510
Illegal NgoMIV site found at 2978
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 285

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 <partinfo>BBa_J45700 short</partinfo>
-This system designed by the MIT 2006 iGEM team generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent).
+We assembled a complete wintergreen odor biosynthetic system (<partinfo>BBa_J45700</partinfo>) by combining the salicylic acid (<partinfo>BBa_J45320</partinfo>) and wintergreen odor generators (<partinfo>BBa_J45120</partinfo>).  <partinfo>BBa_J45700</partinfo> catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor.
+[[Image:BBa_J45700.png|center]]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+To construct a complete wintergreen odor biosynthetic system, we needed to design an additional biosynthetic device for wintergreen odor precursor production.  We designed a biosynthetic device for production of salicylate from the cellular metabolite chorismate (<partinfo>BBa_J45320</partinfo>) based on the ''pchBA'' genes from ''Pseudomonas aeruginosa''.  The enzyme PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate.  The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate.  Serino ''et al.'' previously demonstrated salicylate formation in ''E. coli'' using the ''pchBA'' genes.
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