Difference between revisions of "Part:BBa K2587009"

Revision as of 14:33, 8 October 2018

ptxD_opt

ptxD, from Pseudomonas stutzeri is coding for the phosphonate dehydrogenase, that catalyses the oxidation of phosphite (phosphonate) using NAD+ and H20 to Phosphate and NADH. pH optimum is pH = 7.25-7.75.

Usage and Biology

Most of the microorganisms, especially common used model organsims like Escherichia coli or Saccharomyces cerevisiae grow only very slow on phosphite as phosphorus source. As a result the ptxD gene together with a phosphite media could be used as a selection marker. Without a permanent usage of antibiotics a screening and a decrease of the contamination risk could be achieved.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 93
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 6
Illegal BamHI site found at 1044
Illegal XhoI site found at 216
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 714
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 22
Illegal BsaI.rc site found at 1051

@@ Line 6: / Line 6: @@
 <h4><strong>Usage and Biology</strong></h4>
-<p>Most of the microorganisms, espacially common used modelorgansims like <i>Escherichia coli</i> or <i>Saccharomyces cerevisiae</i> grow only very slow on phosphite as phosphorus source. As a result the ptxD gene together with a phosphite media could be used as a selection marker. Without a permanent usage of antibiotics a screening and a decrease of the contamination risk could be achieved. </p>
+<p>Most of the microorganisms, especially common used model organsims like <i>Escherichia coli</i> or <i>Saccharomyces cerevisiae</i> grow only very slow on phosphite as phosphorus source. As a result the ptxD gene together with a phosphite media could be used as a selection marker. Without a permanent usage of antibiotics a screening and a decrease of the contamination risk could be achieved. </p>
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