Difference between revisions of "Part:BBa K2740020"
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<partinfo>BBa_K2740020 parameters</partinfo> | <partinfo>BBa_K2740020 parameters</partinfo> | ||
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| + | <h2>Parameter of Protein </h2> | ||
| + | <p align="left">Number of amino acids: 378</p> | ||
| + | <p align="left">Molecular weight: 41329.16</p> | ||
| + | <p align="left">Theoretical pI: 5.45</p> | ||
| + | <p align="left">Amino acid composition: <br /> | ||
| + | Ala (A) 45 11.9%<br /> | ||
| + | Arg (R) 25 6.6%<br /> | ||
| + | Asn (N) 9 2.4%<br /> | ||
| + | Asp (D) 19 5.0%<br /> | ||
| + | Cys (C) 5 1.3%<br /> | ||
| + | Gln (Q) 14 3.7%<br /> | ||
| + | Glu (E) 31 8.2%<br /> | ||
| + | Gly (G) 30 7.9%<br /> | ||
| + | His (H) 11 2.9%<br /> | ||
| + | Ile (I) 22 5.8%<br /> | ||
| + | Leu (L) 40 10.6%<br /> | ||
| + | Lys (K) 13 3.4%<br /> | ||
| + | Met (M) 10 2.6%<br /> | ||
| + | Phe (F) 9 2.4%<br /> | ||
| + | Pro (P) 14 3.7%<br /> | ||
| + | Ser (S) 24 6.3%<br /> | ||
| + | Thr (T) 16 4.2%<br /> | ||
| + | Trp (W) 5 1.3%<br /> | ||
| + | Tyr (Y) 9 2.4%<br /> | ||
| + | Val (V) 27 7.1%<br /> | ||
| + | Pyl (O) 0 0.0%<br /> | ||
| + | Sec (U) 0 0.0%</p> | ||
| + | <p align="left"> (B) 0 0.0%<br /> | ||
| + | (Z) 0 0.0%<br /> | ||
| + | (X) 0 0.0%</p> | ||
| + | <p align="left"> </p> | ||
| + | <p align="left">Total number of negatively charged residues (Asp + Glu): 50<br /> | ||
| + | Total number of positively charged residues (Arg + Lys): 38</p> | ||
| + | <p align="left">Atomic composition:</p> | ||
| + | <p align="left">Carbon C 1821<br /> | ||
| + | Hydrogen H 2910<br /> | ||
| + | Nitrogen N 516<br /> | ||
| + | Oxygen O 551<br /> | ||
| + | Sulfur S 15</p> | ||
| + | <p align="left">Formula: C1821H2910N516O551S15<br /> | ||
| + | Total number of atoms: 5813</p> | ||
| + | <p align="left">Extinction coefficients:</p> | ||
| + | <p align="left">Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.</p> | ||
| + | <p align="left">Ext. coefficient 41160<br /> | ||
| + | Abs 0.1% (=1 g/l) 0.996, assuming all pairs of Cys residues form cystines</p> | ||
| + | <p align="left"> </p> | ||
| + | <p align="left">Ext. coefficient 40910<br /> | ||
| + | Abs 0.1% (=1 g/l) 0.990, assuming all Cys residues are reduced</p> | ||
| + | <p align="left">Estimated half-life:</p> | ||
| + | <p align="left">The N-terminal of the sequence considered is M (Met).</p> | ||
| + | <p align="left">The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).<br /> | ||
| + | >20 hours (yeast, in vivo).<br /> | ||
| + | >10 hours (Escherichia coli, in vivo).</p> | ||
| + | <p align="left"> </p> | ||
| + | <p align="left">Instability index:</p> | ||
| + | <p align="left">The instability index (II) is computed to be 43.86<br /> | ||
| + | This classifies the protein as unstable.</p> | ||
| + | <p align="left"> </p> | ||
| + | <p align="left">Aliphatic index: 96.59</p> | ||
| + | <p align="left">Grand average of hydropathicity (GRAVY): -0.087</p> | ||
| + | <div> | ||
| + | <h2>Design Notes</h2> | ||
| + | </div> | ||
| + | <p align="left">Nitrogenase is a complex enzyme system consisting of nine protein components. Additionally, to maintain stoichiometry of these protein components is an essential requirement for nitrogenase biosynthesis and activity. However, there is only one copy of each structure gene present in the nif gene cluster. Therefore, cloning each of these nif genes and setting as independent part can facilitate the regulation of balancing expression ratios from the transcription and/or translation level(s) when they are heterogeneously expressed in non-diazotrophic hosts.</p> | ||
Latest revision as of 10:18, 8 October 2018
CR1 nifV
CR1 nifV encodes homocitrate synthase NifV that enables the synthesis of the homocitrate moiety of the molybdenum-iron protein cofactor.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 378
Molecular weight: 41329.16
Theoretical pI: 5.45
Amino acid composition:
Ala (A) 45 11.9%
Arg (R) 25 6.6%
Asn (N) 9 2.4%
Asp (D) 19 5.0%
Cys (C) 5 1.3%
Gln (Q) 14 3.7%
Glu (E) 31 8.2%
Gly (G) 30 7.9%
His (H) 11 2.9%
Ile (I) 22 5.8%
Leu (L) 40 10.6%
Lys (K) 13 3.4%
Met (M) 10 2.6%
Phe (F) 9 2.4%
Pro (P) 14 3.7%
Ser (S) 24 6.3%
Thr (T) 16 4.2%
Trp (W) 5 1.3%
Tyr (Y) 9 2.4%
Val (V) 27 7.1%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1821
Hydrogen H 2910
Nitrogen N 516
Oxygen O 551
Sulfur S 15
Formula: C1821H2910N516O551S15
Total number of atoms: 5813
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 41160
Abs 0.1% (=1 g/l) 0.996, assuming all pairs of Cys residues form cystines
Ext. coefficient 40910
Abs 0.1% (=1 g/l) 0.990, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.86
This classifies the protein as unstable.
Aliphatic index: 96.59
Grand average of hydropathicity (GRAVY): -0.087
Design Notes
Nitrogenase is a complex enzyme system consisting of nine protein components. Additionally, to maintain stoichiometry of these protein components is an essential requirement for nitrogenase biosynthesis and activity. However, there is only one copy of each structure gene present in the nif gene cluster. Therefore, cloning each of these nif genes and setting as independent part can facilitate the regulation of balancing expression ratios from the transcription and/or translation level(s) when they are heterogeneously expressed in non-diazotrophic hosts.