Difference between revisions of "Part:BBa K2598062"
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<partinfo>BBa_K2598062 parameters</partinfo> | <partinfo>BBa_K2598062 parameters</partinfo> | ||
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| + | ===Characterization=== | ||
| + | <b>Figure 1</b> shows the relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively. We got the data through flow cytometer and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. And every color is E. coli that grows for 8 hours under the light of the corresponding wavelength. We can see E. coli has the highest blue fluorescence expression under blue light from the left graph. And We can also see E. coli has the highest green and red fluorescence expression under green light and right light from the middle and right graph respectively. So this figure proves that our system and our parts can work well. | ||
| + | <div>[[File:T—UCAS-China—LIGHT AND LIGHT.png |1000px|thumb|center|<b>Figure 1:</b>Relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively]]</div> | ||
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| + | ===Characterization=== | ||
| + | <b>Figure 2</b> shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well. | ||
| + | <div>[[File:T—UCAS-China—abc222.png|1000px|thumb|center|<b>Figure 2:</b>Relationship between fluorescence intensity and excitation wavelength]]</div> | ||
Revision as of 09:44, 8 October 2018
smell
This part contains Germacradienol/germacrene D synthase, bmst1 and Limonene synthase under promoters that can be induced by T7 RNAP sigma fragments. We use this part to make our E.coli possess variable scents and bless our rose with unique soul.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1137
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 421
Illegal AgeI site found at 5 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 202
Illegal BsaI site found at 955
Illegal SapI site found at 2924
Characterization
Figure 1 shows the relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively. We got the data through flow cytometer and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. And every color is E. coli that grows for 8 hours under the light of the corresponding wavelength. We can see E. coli has the highest blue fluorescence expression under blue light from the left graph. And We can also see E. coli has the highest green and red fluorescence expression under green light and right light from the middle and right graph respectively. So this figure proves that our system and our parts can work well.
Characterization
Figure 2 shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well.
