Difference between revisions of "Part:BBa K2539300"
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===Characterization=== | ===Characterization=== | ||
AlcR is a transcription factor that, along with substances like ethanol, activates palcA. As part of a functional test testing the efficiency of palcA, this construct was ran as a negative control. | AlcR is a transcription factor that, along with substances like ethanol, activates palcA. As part of a functional test testing the efficiency of palcA, this construct was ran as a negative control. | ||
Revision as of 09:06, 8 October 2018
AlcR Expression Construct
This construct was built to overexpress alcR, a regulatory protein necessary for the activation of palcA (BBa_K2092002). We acquired all parts from the iGEM distribution kit: a strong promoter and strong RBS combination (BBa_K880005) to maximize protein production, strong RBS (BBa_B0034), alcR (BBa_K2092001), and a double terminator (BBa_B0015) to end transcription. PCR checks and sequencing results from Tri-I Biotech confirmed that our final alcR construct is correct.
Characterization
AlcR is a transcription factor that, along with substances like ethanol, activates palcA. As part of a functional test testing the efficiency of palcA, this construct was ran as a negative control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2611
Illegal BamHI site found at 1615
Illegal BamHI site found at 2353 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1319
Illegal AgeI site found at 1447
Illegal AgeI site found at 1978 - 1000COMPATIBLE WITH RFC[1000]