Difference between revisions of "Part:BBa K2539300"

 
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An alcR-based construct that employs the strong promoter/strong RBS (K880005) combination to upregulate expression of alcR, a regulatory protein that, along with substances like ethanol, activates PalcA.
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This construct was built to overexpress alcR, a regulatory protein necessary for the activation of palcA (BBa_K2092002). We acquired all parts from the iGEM distribution kit: a strong promoter and strong RBS combination (BBa_K880005) to maximize protein production, strong RBS (BBa_B0034), alcR (BBa_K2092001), and a double terminator (BBa_B0015) to end transcription. PCR checks and sequencing results from Tri-I Biotech confirmed that our final alcR construct is correct.
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===Usage and Biology===
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===Characterization===
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AlcR is a transcription factor that, along with substances like ethanol, activates palcA. As part of a functional test testing the efficiency of palcA, this construct was ran as a negative control. 
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Revision as of 09:04, 8 October 2018


AlcR Expression Construct

This construct was built to overexpress alcR, a regulatory protein necessary for the activation of palcA (BBa_K2092002). We acquired all parts from the iGEM distribution kit: a strong promoter and strong RBS combination (BBa_K880005) to maximize protein production, strong RBS (BBa_B0034), alcR (BBa_K2092001), and a double terminator (BBa_B0015) to end transcription. PCR checks and sequencing results from Tri-I Biotech confirmed that our final alcR construct is correct.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2611
    Illegal BamHI site found at 1615
    Illegal BamHI site found at 2353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1319
    Illegal AgeI site found at 1447
    Illegal AgeI site found at 1978
  • 1000
    COMPATIBLE WITH RFC[1000]