Difference between revisions of "Part:BBa K2834002"
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===References=== | ===References=== | ||
Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254. | Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 00:30, 8 October 2018
6x His-Tag
Tags are needed in order to purify a recombinant protein, 6x His-tag is one of them. The His-tagged protein can be easily detected and purified using immobilized metal-affinity chromatography (IMAC).
References
Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]