Difference between revisions of "Part:BBa K2834002"
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<partinfo>BBa_K2834002 short</partinfo>
 
<partinfo>BBa_K2834002 short</partinfo>
  
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<partinfo>BBa_K2834002 SequenceAndFeatures</partinfo>
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Defensin 2 has selective activity against Gram-positive bacteria. This peptide disrupts the permeability barrier of the cytoplasmic membrane by forming channels, resulting in a loss of cytoplasmic K+, a partial depolarization of the inner membrane, a decrease in cytoplasmic ATP, and inhibition of respiration processes.
  
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Cociancich, S., Ghazi, A., Hetru, C., Hoffman, J. A., & Letellier, L. (1993). Insect Defensin, an Inducible Antibacterial Peptide, Forms Voltage-dependent Channels in Micrococcus luteus. The Journal of Biological Chemistry, 268(26), 19239-19245
  
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===Design Notes===
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The silent mutations were necessary in order to fulfill IDT's synthesis requirements.
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===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2834002 SequenceAndFeatures</partinfo>
  
  
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===Source===
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===Functional Parameters===
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The sequence was based on another part (BBa_K2342003) but it was modified by adding a stop codon and some silent mutations.
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<partinfo>BBa_K2834002 parameters</partinfo>
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===References===
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Revision as of 00:04, 8 October 2018


6x His-Tag

Defensin 2 has selective activity against Gram-positive bacteria. This peptide disrupts the permeability barrier of the cytoplasmic membrane by forming channels, resulting in a loss of cytoplasmic K+, a partial depolarization of the inner membrane, a decrease in cytoplasmic ATP, and inhibition of respiration processes.

Cociancich, S., Ghazi, A., Hetru, C., Hoffman, J. A., & Letellier, L. (1993). Insect Defensin, an Inducible Antibacterial Peptide, Forms Voltage-dependent Channels in Micrococcus luteus. The Journal of Biological Chemistry, 268(26), 19239-19245

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]