Difference between revisions of "Part:BBa K2871000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The paper described by Charpentier & Oswald (2004), showed that a 20 amino acid sequence was sufficient | + | The paper described by Charpentier & Oswald (2004), showed that a 20 amino acid signal sequence of Map protein was sufficient for translocation via E. coli T3SS. The short length signal sequence seemed attractive to us because we wanted to minimize folding interference between the signal sequence and the intended protein fused to the signal sequence. |
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===Source=== | ===Source=== | ||
Revision as of 19:10, 7 October 2018
Map20, T3SS export signal peptide from Map gene
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 85
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The paper described by Charpentier & Oswald (2004), showed that a 20 amino acid signal sequence of Map protein was sufficient for translocation via E. coli T3SS. The short length signal sequence seemed attractive to us because we wanted to minimize folding interference between the signal sequence and the intended protein fused to the signal sequence.
Source
Synthetic sequence deduced from Amino acid Sequence from the Enteropathogenic E. coli strain E22. Described in the paper Charpentier & Oswald, 2004.
References
Charpentier & Oswald, 2004. journal of baceteriology. 'Identification of the Secretion and Translocation Domain of the Enteropathogenic and Enterohemorrhagic Escherichia coli Effector Cif, Using TEM-1 β-Lactamase as a New Fluorescence-Based Reporter' DOI: 10.1128/JB.186.16.5486-5495.2004 https://jb.asm.org/content/186/16/5486