Difference between revisions of "Part:BBa K2547000"
| Line 5: | Line 5: | ||
This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein. | This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein. | ||
| − | <div style="width:100%" align="center"><"https://static.igem.org/mediawiki/parts/ | + | <div style="width:100%" align="center"><"https://static.igem.org/mediawiki/parts/6/61/T--AHUT_China--_shengwu.jpg"></div> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:52, 7 October 2018
Carbonic anhydrase 2
This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein.
<"
">
">Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]