Difference between revisions of "Part:BBa K2675014"
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<partinfo>BBa_K2675014 short</partinfo> | <partinfo>BBa_K2675014 short</partinfo> | ||
| − | + | This part is a synthetic RBS designed for Tat-SAIRGA ([[Part:BBa_K2675004|BBa_K2675004]]) using the [https://salislab.net/software/forward Salis Lab RBS calculator v2.0] [1, 2]. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This RBS was used to drive the expression of Tat-SAIRGA ([[Part:BBa_K2675004|BBa_K2675004]]) under the control of the ([[Part:BBa_J23100|BBa_J23100]]) promoter in the composite part ([[Part:BBa_K2675044|BBa_K2675044]]). | ||
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| + | Using the Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are: | ||
| + | Translation Initiation Rate (au) : 10020,84 | ||
| + | dG_total (kcal/mol) : -4,65 | ||
| + | dG_mRNA_rRNA (kcal/mol) : -3,12 | ||
| + | dG_spacing (kcal/mol) : 0 | ||
| + | dG_standby (kcal/mol) : 0 | ||
| + | dG_start (kcal/mol) : -2,76 | ||
| + | dG_mRNA (kcal/mol) : -1,49 | ||
| + | Accuracy (warnings) : OK | ||
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Revision as of 13:52, 7 October 2018
Synthetic RBS designed for Tat-SAIRGA
This part is a synthetic RBS designed for Tat-SAIRGA (BBa_K2675004) using the Salis Lab RBS calculator v2.0 [1, 2].
Usage and Biology
This RBS was used to drive the expression of Tat-SAIRGA (BBa_K2675004) under the control of the (BBa_J23100) promoter in the composite part (BBa_K2675044).
Using the Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are: Translation Initiation Rate (au) : 10020,84 dG_total (kcal/mol) : -4,65 dG_mRNA_rRNA (kcal/mol) : -3,12 dG_spacing (kcal/mol) : 0 dG_standby (kcal/mol) : 0 dG_start (kcal/mol) : -2,76 dG_mRNA (kcal/mol) : -1,49 Accuracy (warnings) : OK
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]