Difference between revisions of "Part:BBa K2665011"

Revision as of 09:57, 7 October 2018

TDH3-Mangrin-6xHis-CYC

Mangrin is derived from mangrove and it is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells. This part coded the functional domain(71 amino acids). This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. We used this part for improving halotorelance of yeast.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 842
Illegal BamHI site found at 936
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterization

For the confirmation of the mangrin function of mangrin, we spotted

Reference

[1] A. Yamada, T. Saitoh, T. Mimura et al. (2002) Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance in Escherichia coli, Yeast, and Tobacco Cells, Plant and cell physiology 903-910

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2665011 short</partinfo>
-This part coded functional domain of a salt tolerance protein, Mangrin form mangrove.
+Mangrin is derived from mangrove and it is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells.
-This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts.
+This part coded the functional domain(71 amino acids). This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts.
-Mangrin is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells. We used this part for improving halotorelance of yeast.
+We used this part for improving halotorelance of yeast.
 <!-- Add more about the biology of this part here