Difference between revisions of "Part:BBa K2591013"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | In the field of molecular biology and cell biology, the basic concepts of transcription termination in eukaryotes is widely applied to molecular cloning. For the DNA polymerase II, the transcription termination is always mediated by the process called polyadenylation, which is initiated by poly A signal in the 3’ downstream region of coding sequence. BGH poly A signal is a commonly used terminator in molecular cloning to build a normal expression construct initiated by Pol II. | ||
| + | Cre-loxP recombination system is a widely used system to generate deletions, translocations and inversions at specific site of DNA. It consists of two directional 34bp loxP sequence, when these two loxP sites possess the same direction, the sequence between them will be excised by Cre recombinase, when these two loxP sites possess the opposite direction, the sequence between them will be inverted. This method is implemented both in eukaryotic and prokaryotic cells, particularly, Cre-loxP system is used in transgenic mice as well as neurobiology. Nevertheless, it should have great potential in synthetic biology as a switch. This system is relatively simple compared to other mechanic switch, it only need two LoxP sites and one single Cre enzyme, and then, inversion or deletion happens. | ||
===Characterization=== | ===Characterization=== | ||
Revision as of 07:42, 7 October 2018
LoxP-BGH polyA-LoxP
Usage and Biology
In the field of molecular biology and cell biology, the basic concepts of transcription termination in eukaryotes is widely applied to molecular cloning. For the DNA polymerase II, the transcription termination is always mediated by the process called polyadenylation, which is initiated by poly A signal in the 3’ downstream region of coding sequence. BGH poly A signal is a commonly used terminator in molecular cloning to build a normal expression construct initiated by Pol II. Cre-loxP recombination system is a widely used system to generate deletions, translocations and inversions at specific site of DNA. It consists of two directional 34bp loxP sequence, when these two loxP sites possess the same direction, the sequence between them will be excised by Cre recombinase, when these two loxP sites possess the opposite direction, the sequence between them will be inverted. This method is implemented both in eukaryotic and prokaryotic cells, particularly, Cre-loxP system is used in transgenic mice as well as neurobiology. Nevertheless, it should have great potential in synthetic biology as a switch. This system is relatively simple compared to other mechanic switch, it only need two LoxP sites and one single Cre enzyme, and then, inversion or deletion happens.
Characterization
Application
Reference
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]