Difference between revisions of "Part:BBa K2549002"
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===Biology=== | ===Biology=== | ||
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| − | We thank the authors carefully | + | We thank the authors carefully documented all the sequences. We used these information, reverse translated and performed codon optimization for better expression in human. |
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Revision as of 07:40, 7 October 2018
anti-GFP (LaG2)
Anti-GFP (LaG2) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. It was used by us as an extracellular domain of the SynNotch, and accomplished a contact-dependent signal input against GFP. LaG2 fuses two copies of LaG16 using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity antibody against GFP[1]. This nanobody recognizes our surEGFP (Part:BBa_K2446051) expressing cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Biology
Data from Fridy PC et al, 2014
We thank the authors carefully documented all the sequences. We used these information, reverse translated and performed codon optimization for better expression in human.
Note: Part:BBa_K2549002, Part:BBa_K2549003 and Part:BBa_K2549003 have the same Biology section.
References
- ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170