Difference between revisions of "Part:BBa K2549002"
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===Usage and Biology===
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===Biology===
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=====Data from Fridy PC et al, 2014=====
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We thank the authors carefully document all the sequences reported in Fridy PC et al. We reverse translated and performed codon optimization for better expression in human.
  
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Note: [[Part:BBa_K2549002]], [[Part:BBa_K2549003]] and [[Part:BBa_K2549003]] have the same '''Biology''' section.
  
 
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===References===
 
===References===

Revision as of 07:39, 7 October 2018


anti-GFP (LaG2)

Anti-GFP (LaG2) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. It was used by us as an extracellular domain of the SynNotch, and accomplished a contact-dependent signal input against GFP. LaG2 fuses two copies of LaG16 using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity antibody against GFP[1]. This nanobody recognizes our surEGFP (Part:BBa_K2446051) expressing cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Biology

Data from Fridy PC et al, 2014

We thank the authors carefully document all the sequences reported in Fridy PC et al. We reverse translated and performed codon optimization for better expression in human.

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Note: Part:BBa_K2549002, Part:BBa_K2549003 and Part:BBa_K2549003 have the same Biology section.


References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170