Difference between revisions of "Part:BBa K2794001"
Line 6: Line 6:
 
|-
 
|-
 
|'''Function'''
 
|'''Function'''
−
|Exosome Membrane Anchoring
+
|Exosome Membrane AnchoringE
 
|-
 
|-
 
|'''Use in'''
 
|'''Use in'''
Line 21: Line 21:
 
|}
 
|}
  
 +
==Overview==
  
−
CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family, which means this is a membrane protein that passes through the membrane 4 times.
+
CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker.  
  
−
==Experimental approach==
+
CD63 (varient 1) passes through the membrane 4 times. The N- and C- terminus is both located inside the membrane. The protein has
  
−
==Proof of function==
+
CD63 is recently researched and used in the field of extracellular vesicle (EVs) research. Due to its strong expression on EVs, it is used in exosome identification, extraction and targeted therapy. A protein sequence can be attached to either the N- or C- terminus so that a protein cargo can be loaded into exosomes using CD63. ()
 +
 
 +
==Design==
 +
 
 +
==Experience==
  
 
<!-- -->
 
<!-- -->

Revision as of 06:04, 7 October 2018


CD63 Varient 1 (Homo Sapiens)

CD63 Varient 1 (Homo Sapiens)
Function Exosome Membrane AnchoringE
Use in Mammalian cells
RFC standard RFC 10 compatible
Backbone pSB1C3
Submitted by [http://2018.igem.org/Team:SHSU_China SHSU_China]

Overview

CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker.

CD63 (varient 1) passes through the membrane 4 times. The N- and C- terminus is both located inside the membrane. The protein has

CD63 is recently researched and used in the field of extracellular vesicle (EVs) research. Due to its strong expression on EVs, it is used in exosome identification, extraction and targeted therapy. A protein sequence can be attached to either the N- or C- terminus so that a protein cargo can be loaded into exosomes using CD63. ()

Design

Experience

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References