Difference between revisions of "Part:BBa K2549007:Design"
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===Design Notes===
 
===Design Notes===
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This part is the mouse Notch1 extended regulatory region[1].
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This part is the mouse Notch1 extended regulatory region<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. It was used as the transmembrane core domain of the SynNotch. However, original reported design has a high background activation in transfected 293T cells. We have put a lot of effect in optimizing this critical transmembrane domain for Notch receptor. Comparing with [[Part:BBa_K2549006]], minimal regulatory region (Ile1427 to Arg1752), extended regulatory region (Pro1390 to Arg1752) includes additional '''3''' EGF repeats.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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[1]Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors.
 
−
Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA.
 
−
Cell, 2016 Feb;164(4):780-91  PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012
 

Revision as of 05:21, 7 October 2018


mouse Notch1 core (extended)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 703


Design Notes

This part is the mouse Notch1 extended regulatory region[1]. It was used as the transmembrane core domain of the SynNotch. However, original reported design has a high background activation in transfected 293T cells. We have put a lot of effect in optimizing this critical transmembrane domain for Notch receptor. Comparing with Part:BBa_K2549006, minimal regulatory region (Ile1427 to Arg1752), extended regulatory region (Pro1390 to Arg1752) includes additional 3 EGF repeats.

Source

from IDT(gBlock), codon optimized for human

References

  1. ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012