Difference between revisions of "Part:BBa K2549013"
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<partinfo>BBa_K2549013 short</partinfo> | <partinfo>BBa_K2549013 short</partinfo> | ||
| − | This part is a mutant TEV(tobacco etch virus) protease. The 219 residue of the wild-type TEV protease is mutated from serine to valine to remove autoinactivation and perform a more efficient | + | This part is a mutant TEV (tobacco etch virus) protease. The 219 residue of the wild-type TEV protease is mutated from serine to valine to remove autoinactivation and perform a more efficient cleavage<ref>Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Kapust RB, Tözsér J, Fox JD, ..., Copeland TD, Waugh DS. Protein Eng, 2001 Dec;14(12):993-1000 PMID: 11809930</ref>. TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. We used it to build our IMPLY logic gates. It can also be utilized by other iGEM teams to create their own engineered fusion proteins cleavage system. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2549013 parameters</partinfo> | <partinfo>BBa_K2549013 parameters</partinfo> | ||
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| + | ===References=== | ||
Revision as of 04:42, 7 October 2018
TEV protease
This part is a mutant TEV (tobacco etch virus) protease. The 219 residue of the wild-type TEV protease is mutated from serine to valine to remove autoinactivation and perform a more efficient cleavage[1]. TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. We used it to build our IMPLY logic gates. It can also be utilized by other iGEM teams to create their own engineered fusion proteins cleavage system.
Usage and Biology
TBA
References
- ↑ Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Kapust RB, Tözsér J, Fox JD, ..., Copeland TD, Waugh DS. Protein Eng, 2001 Dec;14(12):993-1000 PMID: 11809930