Difference between revisions of "Part:BBa K2540003"

 
 
(11 intermediate revisions by the same user not shown)
Line 5: Line 5:
 
This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 36709) and is originally found in Acaryochloris marina.  
 
This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 36709) and is originally found in Acaryochloris marina.  
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
The broad host range regulatory element was characterized by Johns <i>et al.</i> among other sequences obtained through metagenomic mining. The relative transcription and translation levels measured by Johns <i> et al. </i> are given below:
 +
 +
<html>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2018/4/4e/T--Rice--HW36709.jpg" width = 70%>
 +
<p><b>Table 1</b>: Relative transcription and translation levels for the broad host range element (strength 8).</p>
 +
<br>
 +
<p> The part was characterized in four <i>E. coli</i> strains, <i>S. oneidensis </i>, and <i>P. putida </i> using mKate2 fluorescent protein. Figure 1 shows fluorescence levels after cells transformed with a plasmid containing mKate2 under the control of the broad host range regulatory element were grown in LB for 24 hours.
 +
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2018/a/ad/T--Rice--HW_str2.png" width = 70%>
 +
<p><b>Figure 1</b>: Fluorescence levels of mKate2 under the control of the broad host range element (strength 8).</p>
 +
 +
</html>
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K2540003 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2540002 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K2540003 parameters</partinfo>
+
<partinfo>BBa_K2540002 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===References===
 +
Johns, N. I. <i>et al.</i>(2018). Metagenomic mining of regulatory elements enables programmable species-selective gene expression. Nature Methods, 15(5), 323–329.

Latest revision as of 03:34, 7 October 2018


Broad host range regulatory element (strength 8)

This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 36709) and is originally found in Acaryochloris marina.

Usage and Biology

The broad host range regulatory element was characterized by Johns et al. among other sequences obtained through metagenomic mining. The relative transcription and translation levels measured by Johns et al. are given below:


Table 1: Relative transcription and translation levels for the broad host range element (strength 8).


The part was characterized in four E. coli strains, S. oneidensis , and P. putida using mKate2 fluorescent protein. Figure 1 shows fluorescence levels after cells transformed with a plasmid containing mKate2 under the control of the broad host range regulatory element were grown in LB for 24 hours.

Figure 1: Fluorescence levels of mKate2 under the control of the broad host range element (strength 8).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 183


References

Johns, N. I. et al.(2018). Metagenomic mining of regulatory elements enables programmable species-selective gene expression. Nature Methods, 15(5), 323–329.