Difference between revisions of "Part:BBa K2549001"
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<partinfo>BBa_K2549001 short</partinfo> | <partinfo>BBa_K2549001 short</partinfo> | ||
| − | Surface-expressed CD19 (surCD19) is built by joining CD8α signal peptide, CD19 extracellular domain and the transmembrane region of PDGFRβ (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. CD8α peptide guides synthesized fusion protein to pass the translocon<ref>https://en.wikipedia.org/wiki/Translocon</ref> into the endoplasmic reticulum<ref>https://en.wikipedia.org/wiki/Endoplasmic_reticulum</ref>, and the fusion protein will be later sugar modified in Golgi<ref>https://en.wikipedia.org/wiki/Golgi_apparatus</ref>, presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFRβ embeds surCD19 on the membrane. It was used as the antigen for [[Part:BBa_K2549005]] | + | Surface-expressed CD19 (surCD19) is built by joining CD8α signal peptide, CD19 extracellular domain and the transmembrane region of PDGFRβ (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. CD8α peptide guides synthesized fusion protein to pass the translocon<ref>https://en.wikipedia.org/wiki/Translocon</ref> into the endoplasmic reticulum<ref>https://en.wikipedia.org/wiki/Endoplasmic_reticulum</ref>, and the fusion protein will be later sugar modified in Golgi<ref>https://en.wikipedia.org/wiki/Golgi_apparatus</ref>, presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFRβ embeds surCD19 on the membrane. It was used as the antigen for [[Part:BBa_K2549005]]. |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 03:06, 7 October 2018
suface-expressed CD19
Surface-expressed CD19 (surCD19) is built by joining CD8α signal peptide, CD19 extracellular domain and the transmembrane region of PDGFRβ (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies[1]. CD8α peptide guides synthesized fusion protein to pass the translocon[2] into the endoplasmic reticulum[3], and the fusion protein will be later sugar modified in Golgi[4], presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFRβ embeds surCD19 on the membrane. It was used as the antigen for Part:BBa_K2549005.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 588
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 757
Illegal SapI site found at 273
Illegal SapI.rc site found at 91
Usage and Biology
Cellular localization
Function as the antigen for Part:BBa_K2549005
References
- ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012
- ↑ https://en.wikipedia.org/wiki/Translocon
- ↑ https://en.wikipedia.org/wiki/Endoplasmic_reticulum
- ↑ https://en.wikipedia.org/wiki/Golgi_apparatus