Difference between revisions of "Part:BBa K2549001"
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<partinfo>BBa_K2549001 short</partinfo>
 
<partinfo>BBa_K2549001 short</partinfo>
  
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Surface-expressed CD19 (surCD19) is built by joining CD8&alpha; signal peptide, CD19 extracellular domain and the transmembrane region of PDGFR&beta; (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. CD8&alpha; peptide guides synthesized fusion protein to pass the translocon<ref>https://en.wikipedia.org/wiki/Translocon</ref> into the endoplasmic reticulum<ref>https://en.wikipedia.org/wiki/Endoplasmic_reticulum</ref>, and the fusion protein will be later sugar modified in Golgi<ref>https://en.wikipedia.org/wiki/Golgi_apparatus</ref>, presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFR&beta; embeds surCD19 on the membrane.
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Surface-expressed CD19 (surCD19) is built by joining CD8&alpha; signal peptide, CD19 extracellular domain and the transmembrane region of PDGFR&beta; (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. CD8&alpha; peptide guides synthesized fusion protein to pass the translocon<ref>https://en.wikipedia.org/wiki/Translocon</ref> into the endoplasmic reticulum<ref>https://en.wikipedia.org/wiki/Endoplasmic_reticulum</ref>, and the fusion protein will be later sugar modified in Golgi<ref>https://en.wikipedia.org/wiki/Golgi_apparatus</ref>, presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFR&beta; embeds surCD19 on the membrane. It was used as the antigen for [[Part:BBa_K2549005]]
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 03:06, 7 October 2018


suface-expressed CD19

Surface-expressed CD19 (surCD19) is built by joining CD8α signal peptide, CD19 extracellular domain and the transmembrane region of PDGFRβ (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies[1]. CD8α peptide guides synthesized fusion protein to pass the translocon[2] into the endoplasmic reticulum[3], and the fusion protein will be later sugar modified in Golgi[4], presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFRβ embeds surCD19 on the membrane. It was used as the antigen for Part:BBa_K2549005

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757
    Illegal SapI site found at 273
    Illegal SapI.rc site found at 91

Usage and Biology

Cellular localization
Function as the antigen for Part:BBa_K2549005

References

  1. ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012
  2. ↑ https://en.wikipedia.org/wiki/Translocon
  3. ↑ https://en.wikipedia.org/wiki/Endoplasmic_reticulum
  4. ↑ https://en.wikipedia.org/wiki/Golgi_apparatus