Difference between revisions of "Part:BBa K2549001"
(surCD19) |
|||
| Line 12: | Line 12: | ||
===== Cellular localization ===== | ===== Cellular localization ===== | ||
| − | ===== Function as the antigen for BBa_K2549005 ===== | + | ===== Function as the antigen for [[Part:BBa_K2549005]] ===== |
| Line 18: | Line 18: | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2549001 parameters</partinfo> | <partinfo>BBa_K2549001 parameters</partinfo> | ||
| − | + | --> | |
Revision as of 02:00, 7 October 2018
suface-expressed CD19
Surface-expressed CD19 (surCD19) is built by joining CD8α signal peptide, CD19 extracellular domain and the transmembrane region of PDGFRβ (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies. CD8α peptide guides synthesized fusion protein to pass the translocon into the endoplasmic reticulum, and the fusion protein will be later sugar modified in Golgi, presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFRβ embeds surCD19 on the membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 588
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 757
Illegal SapI site found at 273
Illegal SapI.rc site found at 91