Difference between revisions of "Part:BBa K2549001"
(surCD19)
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<partinfo>BBa_K2549001 short</partinfo>
 
<partinfo>BBa_K2549001 short</partinfo>
  
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Surface-expressed CD19(surCD19) is built by fusing CD8α signal peptide and CD19 extracellular domain which has a HA tag on its N terminal and a Myc tag on its C terminal to the transmembrane region of PDGFRβ from N terminal to C terminal. CD8α peptide guides surCD19 to be located to the membrane. Transmembrane region of PDGFRβ embeds it on the plasma membrane. The HA tag and the Myc tag facilitate researchers to detect the expression of the surCD19.It was used as one of our antigens to test the activation of SynNotch.
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Surface-expressed CD19 (surCD19) is built by joining CD8&alpha; signal peptide, CD19 extracellular domain and the transmembrane region of PDGFR&beta; (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies. CD8&alpha; peptide guides synthesized fusion protein to pass the [https://en.wikipedia.org/wiki/Translocon translocon] into the [https://en.wikipedia.org/wiki/Endoplasmic_reticulum endoplasmic reticulum], and the fusion protein will be later sugar modified in [https://en.wikipedia.org/wiki/Golgi_apparatus Golgi], presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFR&beta; embeds surCD19 on the membrane.
  
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2549001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2549001 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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===== Cellular localization =====
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===== Function as the antigen for BBa_K2549005 =====
  
  

Revision as of 01:51, 7 October 2018


suface-expressed CD19

Surface-expressed CD19 (surCD19) is built by joining CD8α signal peptide, CD19 extracellular domain and the transmembrane region of PDGFRβ (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies. CD8α peptide guides synthesized fusion protein to pass the translocon into the endoplasmic reticulum, and the fusion protein will be later sugar modified in Golgi, presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFRβ embeds surCD19 on the membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757
    Illegal SapI site found at 273
    Illegal SapI.rc site found at 91

Usage and Biology

Cellular localization
Function as the antigen for BBa_K2549005