Difference between revisions of "Part:BBa Q04401"
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This is a modified version of the tetR inverter <bbpart>Q04400</bbpart> with a single base switch in the RBS:
 
This is a modified version of the tetR inverter <bbpart>Q04400</bbpart> with a single base switch in the RBS:
−
AAAGAGGAGAAA to AAAGAGGGGAAA
+
AAAGAGGAGAAA to AAAGAGGGGAAA. 
  
 
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[[Image:Q04401.JPG|thumb|500px|left|Transfer curve for Q04401 using the Screening Plasmid.  Units are arbitrary. (<bbpart>pSB1A10</bbpart>)]]
+
[[Image:Q04401.JPG|thumb|500px|left|Transfer curve for Q04401 measured in the Screening Plasmid.  Units are arbitrary. (<bbpart>pSB1A10</bbpart>)]]
 
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Revision as of 07:48, 23 February 2008

TetR-derived transcriptional inverter

This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS: AAAGAGGAGAAA to AAAGAGGGGAAA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Transfer curve for Q04401 measured in the Screening Plasmid. Units are arbitrary. (pSB1A10)



Reuse

  • This inverter was successfully reused by the [http://openwetware.org/wiki/IGEM:MIT/2006 2006 MIT iGEM team].
  • Jasonk 02:44, 23 February 2008 (EST):In general, I think it's more likely to work when used on a high-copy (1 series) plasmid. I've tried it on a 3 series, using F2621 as the induction system and did not see flipping of the inverter.