Difference between revisions of "Part:BBa K2788020:Design"
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===Source=== | ===Source=== | ||
| − | This part comes from BBa_K2232003 | + | This part comes from 2017 SZU-China iGEM team's part: BBa_K2232003 |
===References=== | ===References=== | ||
This part created by 2018 SZU-China iGEM team basing on the original part:BBa_K2232003 | This part created by 2018 SZU-China iGEM team basing on the original part:BBa_K2232003 | ||
Revision as of 06:11, 6 October 2018
C125-TupA-6His
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 969
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 789
Illegal BsaI.rc site found at 1181
Design Notes
In order to verify that TupA was indeed expressed at the protein level, 2018SZU-China added a 6-His structure to the original sequence and verified it by western blot.
Source
This part comes from 2017 SZU-China iGEM team's part: BBa_K2232003
References
This part created by 2018 SZU-China iGEM team basing on the original part:BBa_K2232003