Difference between revisions of "Part:BBa K2788020:Design"

 
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===References===
 
===References===
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This part created by 2017 SZU-China iGEM team basing on the original part:BBa_K2232003

Revision as of 06:07, 6 October 2018


C125-TupA-6His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 969
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 789
    Illegal BsaI.rc site found at 1181


Design Notes

In order to verify that TupA was indeed expressed at the protein level, 2018SZU-China added a 6-His structure to the original sequence and verified it by western blot.


Source

This part comes from BBa_K2232003

References

This part created by 2017 SZU-China iGEM team basing on the original part:BBa_K2232003