Difference between revisions of "Part:BBa K2842680"

 
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<partinfo>BBa_K2842680 short</partinfo>
 
<partinfo>BBa_K2842680 short</partinfo>
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[[File:T--UCL--IP RFP GFP gel.png|400px|thumb|left|
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<center>'''Figure 1: BsaI digestions'''</center>
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<p>
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(1) BsaI digested Intein Passenger [https://parts.igem.org/Part:BBa_K2842669 BBa_K2842669] <br />
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(2) BsaI digested RFP inteins [https://parts.igem.org/Part:BBa_K2842680 BBa_K2842680] <br />
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(3) BsaI digested GFP inteins [https://parts.igem.org/Part:BBa_K2842690 BBa_K2842690]
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</p>''' ]]
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Revision as of 15:16, 5 October 2018


Intein Monomer 1: RFP reporter flanked with orthogonal inteins


Figure 1: BsaI digestions

(1) BsaI digested Intein Passenger BBa_K2842669
(2) BsaI digested RFP inteins BBa_K2842680
(3) BsaI digested GFP inteins BBa_K2842690





















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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1165
    Illegal BsaI.rc site found at 28
    Illegal SapI site found at 903
    Illegal SapI.rc site found at 213