Difference between revisions of "Part:BBa K2570002:Design"
(→Source) |
(→Design Notes) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | <span style="font-weight:bold;">Site-directed mutation site </span> | |
| + | TyrB（Pst1：CTGCAG—CTGCAA） | ||
| + | sense primer: GGTGGCTGCAATGCTGAATGACG | ||
| + | |||
| + | Anti-sense primer: TCAGCATTGCAGCCACCACCTG | ||
===Source=== | ===Source=== | ||
Latest revision as of 09:34, 5 October 2018
TyrB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1054
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Site-directed mutation site
TyrB（Pst1：CTGCAG—CTGCAA）
sense primer: GGTGGCTGCAATGCTGAATGACG
Anti-sense primer: TCAGCATTGCAGCCACCACCTG
Source
Phenylalanine aminotransferase Tyrb from Escherichia coli 21B.