Difference between revisions of "Part:BBa P10101:Experience"
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[[File:https://static.igem.org/mediawiki/parts/3/34/35S%2BRTBV_GUS_NosT_results.png|700px|thumb|left|Figure 1) The GUS staining with the upper row of leaves is 35S, and is much stronger than the lower leaves which are using the RTBV promoter instead.]] | [[File:https://static.igem.org/mediawiki/parts/3/34/35S%2BRTBV_GUS_NosT_results.png|700px|thumb|left|Figure 1) The GUS staining with the upper row of leaves is 35S, and is much stronger than the lower leaves which are using the RTBV promoter instead.]] | ||
Revision as of 08:51, 5 October 2018
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how you used this part and how it worked out.
Applications of BBa_P10101
The Cardiff iGEM team of 2018 characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the RTBV promoter using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.
File:Https://static.igem.org/mediawiki/parts/3/34/35S+RTBV GUS NosT results.png
Figure 1) The GUS staining with the upper row of leaves is 35S, and is much stronger than the lower leaves which are using the RTBV promoter instead.
File:Https://static.igem.org/mediawiki/2018/1/19/T--Cardiff Wales--mCherryPromoterAssayQuantified.png
Figure 2) The quantification report of the 35S promoter when linked to mCherry. This shows the raw photon output of leaf tissue in the table below it too.
User Reviews
UNIQb133e3b389ab966a-partinfo-00000000-QINU UNIQb133e3b389ab966a-partinfo-00000001-QINU