Difference between revisions of "Part:BBa K2549012:Design"

 
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===References===
 
===References===
 +
[1]A synthetic biology framework for programming eukaryotic transcription functions.
 +
Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ.
 +
Cell, 2012 Aug;150(3):647-58  PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045
 +
 +
[2]A tunable zinc finger-based framework for Boolean logic computation in mammalian cells.
 +
Lohmueller JJ, Armel TZ, Silver PA.
 +
Nucleic Acids Res, 2012 Jun;40(11):5180-7  PMID: 22323524; DOI: 10.1093/nar/gks142
 +
 +
[3]A promiscuous split intein with expanded protein engineering applications.
 +
Stevens AJ, Sekar G, Shah NH, ..., Cowburn D, Muir TW.
 +
Proc Natl Acad Sci U S A, 2017 Aug;114(32):8538-8543  PMID: 28739907; DOI: 10.1073/pnas.1701083114

Revision as of 08:47, 5 October 2018


ZF21.16 split C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid residues of the recognition helices for three-finger arrays are substituted by the reported synthetic zinc finger 21.16 residues[1] on the basis of the BCR_ABL-1 artificial zinc finger[2].


Source

IDT(gBlock)

References

[1]A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58 PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045

[2]A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Lohmueller JJ, Armel TZ, Silver PA. Nucleic Acids Res, 2012 Jun;40(11):5180-7 PMID: 22323524; DOI: 10.1093/nar/gks142

[3]A promiscuous split intein with expanded protein engineering applications. Stevens AJ, Sekar G, Shah NH, ..., Cowburn D, Muir TW. Proc Natl Acad Sci U S A, 2017 Aug;114(32):8538-8543 PMID: 28739907; DOI: 10.1073/pnas.1701083114