Difference between revisions of "Part:BBa K2555002"
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<partinfo>BBa_K2555002 short</partinfo>
 
<partinfo>BBa_K2555002 short</partinfo>
  
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This construct contains pBAD (L-arabinose inducing promoter) with RBS and CueR (a transcription factor that binds onto pCopA). CueR under the control of the promoter it regulates would produce a positive feedback loop, making the construct more sensitive to different copper concentrations.
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This construct contains pBAD (L-arabinose inducing promoter) with RBS and CueR (a transcription factor that binds onto pCopA). E. coli cells use  CueR to regulate the cytoplasmic copper concentration. Under different copper concentrations, CueR behaves as a net activator or a net repressor. Arabinose concentation controls the expression of Cue R in the construct. This part is used for construction of BBa_K2555004.
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CueR can act as both a positive and negative regulator and our results seem to indicate that negative regulation may be occurring here.
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K2555002 parameters</partinfo>
 
<partinfo>BBa_K2555002 parameters</partinfo>
 
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<p>References</p>
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<p>(1)Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472.</P>

Revision as of 14:15, 4 October 2018


pBAD-RBS-CueR

This construct contains pBAD (L-arabinose inducing promoter) with RBS and CueR (a transcription factor that binds onto pCopA). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Under different copper concentrations, CueR behaves as a net activator or a net repressor. Arabinose concentation controls the expression of Cue R in the construct. This part is used for construction of BBa_K2555004.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1067


References

(1)Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472.