Difference between revisions of "Part:BBa K2812006:Design"

Revision as of 12:56, 4 October 2018

Coding sequence for Pyocin S5 with HlyA and His6-tag

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 137
Illegal BglII site found at 425
Illegal BglII site found at 2110
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1200

Design Notes

DNA sequence codon optimized for E. coli.

Source

Organism of origin: Pseudomonas aeruginosa PAO1. Sample source: Synthesized by IDT

References

Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.

Revision as of 12:52, 4 October 2018 (view source) MariskaBruls (Talk \| contribs) (→‎Source) ← Older edit		Revision as of 12:56, 4 October 2018 (view source) MariskaBruls (Talk \| contribs) m (→‎References) Newer edit →
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	===References===		===References===
		+
		+	Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.