Difference between revisions of "Part:BBa K2740019"
| Line 17: | Line 17: | ||
<partinfo>BBa_K2740019 parameters</partinfo> | <partinfo>BBa_K2740019 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | <h2>Parameter of Protein </h2> | ||
| + | <p align="left">Number of amino acids: 254</p> | ||
| + | <p align="left">Molecular weight: 27871.11</p> | ||
| + | <p align="left">Theoretical pI: 5.42</p> | ||
| + | <p align="left">Amino acid composition: <br /> | ||
| + | Ala (A) 25 9.8%<br /> | ||
| + | Arg (R) 19 7.5%<br /> | ||
| + | Asn (N) 8 3.1%<br /> | ||
| + | Asp (D) 12 4.7%<br /> | ||
| + | Cys (C) 8 3.1%<br /> | ||
| + | Gln (Q) 8 3.1%<br /> | ||
| + | Glu (E) 19 7.5%<br /> | ||
| + | Gly (G) 26 10.2%<br /> | ||
| + | His (H) 6 2.4%<br /> | ||
| + | Ile (I) 14 5.5%<br /> | ||
| + | Leu (L) 27 10.6%<br /> | ||
| + | Lys (K) 5 2.0%<br /> | ||
| + | Met (M) 13 5.1%<br /> | ||
| + | Phe (F) 3 1.2%<br /> | ||
| + | Pro (P) 12 4.7%<br /> | ||
| + | Ser (S) 9 3.5%<br /> | ||
| + | Thr (T) 13 5.1%<br /> | ||
| + | Trp (W) 3 1.2%<br /> | ||
| + | Tyr (Y) 9 3.5%<br /> | ||
| + | Val (V) 15 5.9%<br /> | ||
| + | Pyl (O) 0 0.0%<br /> | ||
| + | Sec (U) 0 0.0%</p> | ||
| + | <p align="left"> (B) 0 0.0%<br /> | ||
| + | (Z) 0 0.0%<br /> | ||
| + | (X) 0 0.0%</p> | ||
| + | <p align="left"> </p> | ||
| + | <p align="left">Total number of negatively charged residues (Asp + Glu): 31<br /> | ||
| + | Total number of positively charged residues (Arg + Lys): 24</p> | ||
| + | <p align="left">Atomic composition:</p> | ||
| + | <p align="left">Carbon C 1212<br /> | ||
| + | Hydrogen H 1941<br /> | ||
| + | Nitrogen N 347<br /> | ||
| + | Oxygen O 364<br /> | ||
| + | Sulfur S 21</p> | ||
| + | <p align="left">Formula: C1212H1941N347O364S21<br /> | ||
| + | Total number of atoms: 3885</p> | ||
| + | <p align="left">Extinction coefficients:</p> | ||
| + | <p align="left">Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.</p> | ||
| + | <p align="left">Ext. coefficient 30410<br /> | ||
| + | Abs 0.1% (=1 g/l) 1.091, assuming all pairs of Cys residues form cystines</p> | ||
| + | <p align="left"> </p> | ||
| + | <p align="left">Ext. coefficient 29910<br /> | ||
| + | Abs 0.1% (=1 g/l) 1.073, assuming all Cys residues are reduced</p> | ||
| + | <p align="left">Estimated half-life:</p> | ||
| + | <p align="left">The N-terminal of the sequence considered is M (Met).</p> | ||
| + | <p align="left">The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).<br /> | ||
| + | >20 hours (yeast, in vivo).<br /> | ||
| + | >10 hours (Escherichia coli, in vivo).</p> | ||
| + | <p align="left"> </p> | ||
| + | <p align="left">Instability index:</p> | ||
| + | <p align="left">The instability index (II) is computed to be 44.28<br /> | ||
| + | This classifies the protein as unstable.</p> | ||
| + | <p align="left"> </p> | ||
| + | <p align="left">Aliphatic index: 89.92</p> | ||
| + | <p align="left">Grand average of hydropathicity (GRAVY): -0.088</p> | ||
| + | <div> | ||
| + | <h2>Design Notes</h2> | ||
| + | </div> | ||
| + | <p align="left">Nitrogenase is a complex enzyme system consisting of nine protein components. Additionally, to maintain stoichiometry of these protein components is an essential requirement for nitrogenase biosynthesis and activity. However, there is only one copy of each structure gene present in the nif gene cluster. Therefore, cloning each of these nif genes and setting as independent part can facilitate the regulation of balancing expression ratios from the transcription and/or translation level(s) when they are heterogeneously expressed in non-diazotrophic hosts.</p> | ||
Revision as of 05:35, 4 October 2018
CR1 hesA
CR1 hesA encodes a NAD/FAD-binding protein, HesA, which is involved in molybdopterin and thiamine biosynthesis. It plays an important role in nitrogen fixation and is responsive to the availability of molybdenum.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 286
Parameter of Protein
Number of amino acids: 254
Molecular weight: 27871.11
Theoretical pI: 5.42
Amino acid composition:
Ala (A) 25 9.8%
Arg (R) 19 7.5%
Asn (N) 8 3.1%
Asp (D) 12 4.7%
Cys (C) 8 3.1%
Gln (Q) 8 3.1%
Glu (E) 19 7.5%
Gly (G) 26 10.2%
His (H) 6 2.4%
Ile (I) 14 5.5%
Leu (L) 27 10.6%
Lys (K) 5 2.0%
Met (M) 13 5.1%
Phe (F) 3 1.2%
Pro (P) 12 4.7%
Ser (S) 9 3.5%
Thr (T) 13 5.1%
Trp (W) 3 1.2%
Tyr (Y) 9 3.5%
Val (V) 15 5.9%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1212
Hydrogen H 1941
Nitrogen N 347
Oxygen O 364
Sulfur S 21
Formula: C1212H1941N347O364S21
Total number of atoms: 3885
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 30410
Abs 0.1% (=1 g/l) 1.091, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 1.073, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.28
This classifies the protein as unstable.
Aliphatic index: 89.92
Grand average of hydropathicity (GRAVY): -0.088
Design Notes
Nitrogenase is a complex enzyme system consisting of nine protein components. Additionally, to maintain stoichiometry of these protein components is an essential requirement for nitrogenase biosynthesis and activity. However, there is only one copy of each structure gene present in the nif gene cluster. Therefore, cloning each of these nif genes and setting as independent part can facilitate the regulation of balancing expression ratios from the transcription and/or translation level(s) when they are heterogeneously expressed in non-diazotrophic hosts.