Difference between revisions of "Part:BBa K2570011"
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In our design, our engineering bacteria usually put in a spray container and store at room temperature. When temperature stays below 37 degree, the temperature promoter will not work.  
 
In our design, our engineering bacteria usually put in a spray container and store at room temperature. When temperature stays below 37 degree, the temperature promoter will not work.  
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[[File:T--FJNU-China--room_temperature.gif|600px|thumb|center|Fig.1 Compared with the negative control (the empty pSB1C3 vector), OD600 of the two circuits containing toxin proteins are obviously lower, and the difference is evident as time going on. No obvious difference observed among the three groups with no induction. It showed that toxin proteins didn’t leak out.]]
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[[File:https://static.igem.org/mediawiki/2018/d/d0/T--FJNU-China--room_temperature.gif|600px|thumb|center|Fig.1 Compared with the negative control (the empty pSB1C3 vector), OD600 of the two circuits containing toxin proteins are obviously lower, and the difference is evident as time going on. No obvious difference observed among the three groups with no induction. It showed that toxin proteins didn’t leak out.]]
 
<p  style="width:20%;"><img src="https://static.igem.org/mediawiki/2018/d/d0/T--FJNU-China--room_temperature.gif"></p>
 
<p  style="width:20%;"><img src="https://static.igem.org/mediawiki/2018/d/d0/T--FJNU-China--room_temperature.gif"></p>
  

Revision as of 05:30, 4 October 2018


dsrA+lacI+PlacI+hns+ProU+GFP

In our project, we combined temperature promoter dsrA and salt-concentration promoter ProU as a logical gate controlled by salt-concentration and temperature to realize the aim of producing antibacterial, PLA. dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher. [1]The salt-concentration promoter ProU provides sensitivity to the salt concentration to human skin. ProU is a promoter existed in E.coli, induced by low salt concentration. And the HNS is a global-regulated protein which could bind to genome and specifically inhibit ProU.[2]

In our design, our engineering bacteria usually put in a spray container and store at room temperature. When temperature stays below 37 degree, the temperature promoter will not work.

File:Https://static.igem.org/mediawiki/2018/d/d0/T--FJNU-China--room temperature.gif
Fig.1 Compared with the negative control (the empty pSB1C3 vector), OD600 of the two circuits containing toxin proteins are obviously lower, and the difference is evident as time going on. No obvious difference observed among the three groups with no induction. It showed that toxin proteins didn’t leak out.

<img src="T--FJNU-China--room_temperature.gif">

The function of PlacI has became a constitutive promoter. The global regulate protein HNS will be express to inhibit proU. Then the logical gate will be “off” state. On the other hand, when we use the spray on the skin, temperature reached 37 degree, the repressor protein lacI will be expressed to inhibit PlacI, the HNS won’t be revealed. Then, once people sweat, the Na+ and Cl- will induce proU, the logical gate became “on” state.

<img src="T--FJNU-China--on_skin.gif">

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1587
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3324
    Illegal SapI.rc site found at 154
    Illegal SapI.rc site found at 1973