Difference between revisions of "Part:BBa K2865002"

Latest revision as of 03:08, 4 October 2018

[AAV9]-Left-ITR

The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle vectors. They are named so because of their symmetry, which has been shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle vectors respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the left ITR of AAV-9.

Usage and Biology

The AAV genome is built of single-stranded DNA, which is about 4.7 kilobase long. ITRs play a very important role in the AAV genome and are regarded as the only cis-acting element in AAV replication and encapsidation. Their hairpin structure, providing DNA polymerase with free-OH group at the 3' end, allows primase-independent synthesis of the second DNA strand. The ITRs were also shown to be required for efficient encapsidation of the AAV DNA combined with generation of a fully assembled, deoxyribonuclease-resistant AAV particles.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2865002 short</partinfo>
-The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle plasmids. They are named so because of their symmetry, which was shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle plasmid respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the left ITR of AAV-9.
+The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle vectors. They are named so because of their symmetry, which has been shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle vectors respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the left ITR of AAV-9.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+The AAV genome is built of single-stranded DNA, which is about 4.7 kilobase long. ITRs play a very important role in the AAV genome and are regarded as the only cis-acting element in AAV replication and encapsidation. Their hairpin structure, providing DNA polymerase with free-OH group at the 3' end, allows primase-independent synthesis of the second DNA strand. The ITRs were also shown to be required for efficient encapsidation of the AAV DNA combined with generation of a fully assembled, deoxyribonuclease-resistant AAV particles.
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