Difference between revisions of "Part:BBa K2587009:Design"
 
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===Design Notes===
 
===Design Notes===
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For codon optimisation for S.cerevisiae, arginine codons (CGC, CGA, CGG) and an alanine codon (GCG) of ptxD were changed to (AGA and GCT, respectively) (Kanda et al., 2014). Overhangs for golden gate cloning with YTK Toolbox of Lee et al in 2015 were added.
+
For codon optimisation for <i>S.cerevisiae</i>, arginine codons (CGC, CGA, CGG) and an alanine codon (GCG) of ptxD were changed to (AGA and GCT, respectively) (Kanda et al., 2014). Overhangs for golden gate cloning with YTK Toolbox of Lee et al in 2015 were added.
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+
−
https://static.igem.org/mediawiki/parts/a/af/Plasmidcard_pYTK001_ptxDopt.png
+
  
 +
https://static.igem.org/mediawiki/parts/f/fd/Plasmidcard_pSCB13_ptxDopt_map.png
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<p><h4><strong>Figure 1:</strong></h4> Plasmidcard showing the ptxDopt basic part in the iGEM-backbone</p>
 
===Source===
 
===Source===
  
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===References===
 
===References===
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<p>Kanda, Keisuke, et al. "Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts." Journal of biotechnology 182 (2014): 68-73.:<br>
 +
https://www.sciencedirect.com/science/article/pii/S016816561400193X</p>
 +
Lee, Michael E., et al. "A highly characterized yeast toolkit for modular, multipart assembly." ACS synthetic biology 4.9 (2015): 975-986.:<br>
 +
https://pubs.acs.org/doi/abs/10.1021/sb500366v

Latest revision as of 17:20, 3 October 2018


ptxD_opt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6
    Illegal BamHI site found at 1044
    Illegal XhoI site found at 216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 22
    Illegal BsaI.rc site found at 1051


Design Notes

For codon optimisation for S.cerevisiae, arginine codons (CGC, CGA, CGG) and an alanine codon (GCG) of ptxD were changed to (AGA and GCT, respectively) (Kanda et al., 2014). Overhangs for golden gate cloning with YTK Toolbox of Lee et al in 2015 were added.

Plasmidcard_pSCB13_ptxDopt_map.png

Figure 1:

Plasmidcard showing the ptxDopt basic part in the iGEM-backbone

Source

synthesized codon optimized by IDT.

References

Kanda, Keisuke, et al. "Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts." Journal of biotechnology 182 (2014): 68-73.:
https://www.sciencedirect.com/science/article/pii/S016816561400193X

Lee, Michael E., et al. "A highly characterized yeast toolkit for modular, multipart assembly." ACS synthetic biology 4.9 (2015): 975-986.:
https://pubs.acs.org/doi/abs/10.1021/sb500366v