Difference between revisions of "Part:BBa K2587009:Design"

Revision as of 17:16, 3 October 2018

ptxD_opt

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 93
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 6
Illegal BamHI site found at 1044
Illegal XhoI site found at 216
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 714
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 22
Illegal BsaI.rc site found at 1051

Design Notes

For codon optimisation for S.cerevisiae, arginine codons (CGC, CGA, CGG) and an alanine codon (GCG) of ptxD were changed to (AGA and GCT, respectively) (Kanda et al., 2014). Overhangs for golden gate cloning with YTK Toolbox of Lee et al in 2015 were added.

Figure 1:

Plasmidcard showing the ptxDopt basic part in the iGEM-backbone

Source

synthesized codon optimized by IDT.

References

Kanda, Keisuke, et al. "Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts." Journal of biotechnology 182 (2014): 68-73.: https://www.sciencedirect.com/science/article/pii/S016816561400193X Lee, Michael E., et al. "A highly characterized yeast toolkit for modular, multipart assembly." ACS synthetic biology 4.9 (2015): 975-986.: https://pubs.acs.org/doi/abs/10.1021/sb500366v

@@ Line 16: / Line 16: @@
 ===References===
-Kanda et al., 2014:<a href="https://www.sciencedirect.com/science/article/pii/S016816561400193X"> (Kanda et al., 2014)</a>
+Kanda, Keisuke, et al. "Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts." Journal of biotechnology 182 (2014): 68-73.:
+https://www.sciencedirect.com/science/article/pii/S016816561400193X
+Lee, Michael E., et al. "A highly characterized yeast toolkit for modular, multipart assembly." ACS synthetic biology 4.9 (2015): 975-986.:
+https://pubs.acs.org/doi/abs/10.1021/sb500366v