Difference between revisions of "Part:BBa K2587027"
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This construct comprises parts from the Quorum sensing system from Vibrio fischeri. It contains the LuxI, the acyl homoserine lactone synthase, the LuxR, the regulator, which will bind to the promoter pLux, that will induce synthesis of GFP.  
 
This construct comprises parts from the Quorum sensing system from Vibrio fischeri. It contains the LuxI, the acyl homoserine lactone synthase, the LuxR, the regulator, which will bind to the promoter pLux, that will induce synthesis of GFP.  
  
−
<h4><strong>Usage and Biology</strong></h4>
 
  
 +
<strong><h4>Usage and Biology</h4></strong>
 +
<br>
 +
<p><ul>
 +
<li> <strong> Acyl homoserine lactone synthase</strong>: LuxI</li>
 +
<li> <strong>Regulator</strong>: LuxR</li>
 +
<li> <strong>Promoter</strong>: pLux, inducible by the quorum sensing molecule acyl homoserine lactone bound to LuxR</li>
 +
<li> <strong>GFP</strong>fluorescent protein</li>
 +
<li> GFP expression is induced after synthesis of the acyl homerine lactone by LuxI, which binds with LuxR to the pLux promoter </li>
 +
<li> This construct shows functionality of promoter by increase of fluorescence upon time</li>
 +
<li> This part contains Type II S restriction sites and can be further used for scarless assembly with other parts</li>
 +
</ul>
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</p>
  
 
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Revision as of 16:27, 3 October 2018


luxI_luxR_Plux_gfp

This construct comprises parts from the Quorum sensing system from Vibrio fischeri. It contains the LuxI, the acyl homoserine lactone synthase, the LuxR, the regulator, which will bind to the promoter pLux, that will induce synthesis of GFP.


Usage and Biology


  • Acyl homoserine lactone synthase: LuxI
  • Regulator: LuxR
  • Promoter: pLux, inducible by the quorum sensing molecule acyl homoserine lactone bound to LuxR
  • GFPfluorescent protein
  • GFP expression is induced after synthesis of the acyl homerine lactone by LuxI, which binds with LuxR to the pLux promoter
  • This construct shows functionality of promoter by increase of fluorescence upon time
  • This part contains Type II S restriction sites and can be further used for scarless assembly with other parts

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 829
    Illegal NheI site found at 852
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 892
    Illegal BsaI site found at 1681
    Illegal BsaI.rc site found at 1523
    Illegal BsaI.rc site found at 1790
    Illegal BsaI.rc site found at 2468