Difference between revisions of "Part:BBa K2599003"

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Antimicrobial peptide - Enterocin B

This biobrick is the basic part of the enterocin B, more information please look for the composite part [1].

Introduction

Same as enterocin A, enterocin B is also from Enterococcus faecium that contains disulfide bridge. However, enterocin B does not belong to the pediocin family of bacteriocins. The primary translation product was a 71 aa peptide containing a leader peptide, and it is found to be a heat-stable bacteriocins, which can synergistically act with enterocin A.

Mechanism of Enterocin B

The bacteriocins inhibit their target organisms through pore formation. Though the mechanism of each inhibition is vary from species to species, the general process is conserved. To see more details, please search for our project page.

Since enterocin B belongs to class II bacteriocin, it is a small and heat stable peptide with a narrow spectrum of activity. Also, Enterocin A and enterocin B have very similar inhibitory spectra. They both interact with anionic lipids that are abundantly present in the membranes of Gram-positive bacteria. And according to the reference, enterocin B is inhibitory to some strains that are not sensitive to enterocin A. Furthermore, enterocin B is able to kill the bacteria that survive from the enterocin A treatment.

Features of Enterocin B

1. Species Specific

Bacteriocins are antimicrobial peptides that will kill or inhibit bcterial strains closely related or non-related to produced bacteria, but will not harm the bacteria themselves by specific immunity proteins. The organisims that Enterocin B targets including Enterococcus faecalis, Bacillus subtilis, Bacillus coagulans, etc. More target organisms can be found on [http://bactibase.hammamilab.org/BAC101 bactibase].

2. Eco-friendly

Since enterocin B is a polypeptide naturally produced by bacteria itself and can inhibit other bacteria without much environment impact. It don't pose threat to other organisms like farm animals or humans. Therefore, this toxin will not cause safety problem.

3. Biodegradable

Enterocin B is a short peptide that will degrade in a short time. After degradation, this antibacterial peptide is harmless to our environment.

Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The enterocin B sequence length is around 210 b.p.

Sequence and Features

Reference