Difference between revisions of "Part:BBa K2587009:Design"
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For codon optimisation for S.cerevisiae, arginine codons (CGC, CGA, CGG) and an alanine codon (GCG) of ptxD were changed to (AGA and GCT, respectively) (Kanda et al., 2014). Overhangs for golden gate cloning with YTK Toolbox of Lee et al in 2015 were added.
 
For codon optimisation for S.cerevisiae, arginine codons (CGC, CGA, CGG) and an alanine codon (GCG) of ptxD were changed to (AGA and GCT, respectively) (Kanda et al., 2014). Overhangs for golden gate cloning with YTK Toolbox of Lee et al in 2015 were added.
  
−
https://static.igem.org/mediawiki/parts/a/af/Plasmidcard_pYTK001_ptxDopt.png
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https://static.igem.org/mediawiki/parts/f/fd/Plasmidcard_pSCB13_ptxDopt_map.png
  
 
===Source===
 
===Source===

Revision as of 09:42, 2 October 2018


ptxD_opt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6
    Illegal BamHI site found at 1044
    Illegal XhoI site found at 216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 22
    Illegal BsaI.rc site found at 1051


Design Notes

For codon optimisation for S.cerevisiae, arginine codons (CGC, CGA, CGG) and an alanine codon (GCG) of ptxD were changed to (AGA and GCT, respectively) (Kanda et al., 2014). Overhangs for golden gate cloning with YTK Toolbox of Lee et al in 2015 were added.

Plasmidcard_pSCB13_ptxDopt_map.png

Source

synthesized codon optimized by IDT.

References